|
KMID : 0380619860180040288
|
|
Korean Journal of Food Science and Technology
1986 Volume.18 No. 4 p.288 ~ p.293
|
|
|
Purification and Characterization of Raw Starch - Digesting Enzyme from Rhizopus oryzae
|
|
|
|
|
Abstract
|
|
|
|
A raw starch-digesting enzyme from Rhimpus oryzae was purified by ammonium sulfate fractionation, DEAEsephacel column chromatography and Sephadex G-150 gel filtration. The specific activity of purified enzyme was 45.2 U/㎎ protein and the yield was 16.2%. The purified enzyme was found to be homogeneous by polyacrylamide gel electrophoresis and its molecular weight was estimated to be 67,000 by SDS-polyacrylamide gel electrophoresis, and also the enzyme had Km value of 4.082 ㎎/㎖ for raw corn starch. The optimal temperature and pH for the enzyme activity were 50℃ and 4.0-5.0, respectively. Reaction product of raw corn starch by purified enzyme was glucose mainly.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|